The distance traveled by the light through the sample is known as path length. The length between the inner walls of a UV fish bucket where light passes is known as the path of light. The internal distance between the front and rear windows of a conventional spectrophotometer box is the path of the light or the uv cuvettes length of the path. Usually a UV fish bucket is chosen with exact dimensions, such as a path length of 10 mm. Uv vis cuvette for spectrophotometry is transparent for the wavelength of light in the UV fish ranges for measurements. As a result, the bucket itself does not cause problems during the examination.
It has the highest transmission and temperature resistance, the main thing is that it is transparent in both visible light and UV range and is a suitable choice when measuring samples in the UV light spectrum. Of course, there are some exceptions to the rule and some buckets must be filled to 100% in order to function properly. These buckets are our sub-micro buckets, and any kind of current through the cell. When the sample sizes are as small as uL, make sure there is enough sample in the bucket to allow light to pass through.
If it is not prescribed by the nature of the application, then the material of the bucket provides an additional choice that must be made. In general, glass buckets show greater transparency and measurement accuracy and can be reused many times. Since plastic buckets are used only once and do not need to be cleaned, there is no need to take into account possible damage and loss. For these reasons, certain buckets, such as macro buckets, offer an advantage in temperature-controlled applications. Fluorescence buckets are buckets with 4 light walls, while absorption buckets are usually 2 clear-walled ones.
Budget is an important consideration when selecting laboratory equipment or solutions. Plastic is the most cost-effective bucket of the available options. Various transparent materials, such as optical glass, quartz or transparent plastic, are widely used to make buckets.
For experiments that require high purity, disposable plastic buckets are preferred, as they minimize the risk of contamination. But for the use of organic solvents, glass buckets are selected, as they show greater resistance to solvents compared to plastic. Disposable plastic buckets are suitable for research that requires high purity, as they limit the chances of contamination.
Whichever wavelength selector is used in the spectrophotometer, light passes through a sample. For all analyses, it is necessary to measure a reference sample, often called a “blank sample”, such as a bucket filled with a similar solvent used to prepare the sample. If an aqueous buffered solution with the sample is used for measurements, the aqueous buffer solution shall be used as a reference without the relevant substance. When examining bacterial cultures, sterile culture media would be used as a reference. The reference sample signal is then automatically used by the instrument to obtain the actual absorption values of the analytes.
The material in the bucket can cause its own absorption, depending on the wavelength. Quartz semimers are fully translucent over the entire measuring range (UV/Fish). Disposable plastic buckets are often only translucent in the visible range of the spectrum. Therefore, the selection of the bucket depends mainly on the application. ECuvettes produces and develops a complete range of bottles and cells made of optical glass, quartz, NIR and sapphire for laboratory use. Custom design of buckets and cells in different sizes or materials.
In general, quartz and glass buckets have greater transmission and accuracy of spectroscopy measurements, and these buckets can be reused much more often. However, the plastic bucket is cheap and easy to use, without the need to clean and prevent cross-contamination, making it an excellent choice for proteins, DNA and RNA and aqueous solutions. Standard buckets with a path length of 10 mm are most commonly used for most solution analyses. However, there is a chance that the samples are too diluted and it is not possible to obtain sufficient absorption with standard-sized buckets.
For solutions with absorption coefficients that are too high for a standard path length bucket, we have buckets with trajectory lengths as short as 1 mm. You can achieve a tenfold reduction in absorption by simply replacing buckets, instead of wasting time and solvent diluting your solutions. Absorption or transmission determinations shine the light from the spectrometer directly through the bucket to measure the amount of light absorbed or transmitted by the sample. In this measurement configuration, the walls of the bucket on the other side must be optically “clear” in order for light to pass through the sample unhindered. The other two sides of the bucket are often “matte” to allow cell treatment without polluting the optical surfaces of the other two “measuring sides” of the bucket. Sapphire buckets are the most expensive, although they offer the most durable, scratch-resistant and transferable material.